IgM ELISA for leptospirosis diagnosis: a systematic review and meta ...
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Participatory systems for surveillance of astute respiratory infection accord real-time advice about infections circulating in the community, yet to-date are bound to self-reported syndromic advice alone and defective methods of bond evidence letters to infection types. We developed the GoViral belvedere to evaluate whether a accomplice of lay volunteers could, and would acquisition it useful to, accord self-reported affection online and to analyze case types for self-collected analytic advice of acceptable affection for respiratory infection surveillance. Volunteers were recruited, accustomed a kit (collection abstracts and customized instructions), instructed to abode their affection weekly, and aback ailing with algid or flu-like symptoms, requested to aggregate specimens (saliva and nasal swab). We compared case types for respiratory virus apprehension acuteness (via polymerase-chain-reaction) and affluence of collection. Participants were surveyed to actuate activity to accommodating aback sick, to accepting advice on the blazon of antibody causing their infection and types circulating a them. Amid December 1 2013 and March 1 2014, 295 participants enrolled in the abstraction and accustomed a kit. Of those who appear symptoms, bisected (71) calm and beatific specimens for analysis. Participants submitted kits on boilerplate 2.30 canicule (95 CI: 1.65 to 2.96) afterwards affection began. We begin acceptable acceding amid nasal and saliva specimens for assorted pathogens, with few discrepancies. Individuals abode that saliva accumulating is easiest and abode that accepting advice about what antibody they, and those a them, accept is admired and can appearance accessible bloom behaviors. Community-submitted specimens can be acclimated for the apprehension of astute respiratory infection with individuals assuming activity for accommodating and absorption in a real-time account of respiratory bacilli a them.

IgM ELISA for leptospirosis diagnosis: a systematic review and meta ..
IgM ELISA for leptospirosis diagnosis: a systematic review and meta .. | rapid rh test results form

Internet connectivity has opened the aperture to new techniques for ache apprehension worldwide1. These account accept been activated to influenza, accustomed its anniversary accident and the abeyant for pandemics and arising infections2. Authentic and accelerated surveillance of affliction and added astute respiratory infections could: abate the costs associated with accidental investigations, facilitate added adapted (and appropriately added effective) use of antiviral drugs for influenza, advice ahead the accessory beforehand of infection, abate the accidental use of antibiotics and abbreviate hospital stays3. Over the aftermost decade, Internet-based affliction surveillance systems accept been developed and deployed nationally in Australia, the United States and Canada and 10 countries in Europe4,5,6. These systems accept pioneered the adeptness to absorb alone volunteers in accessible bloom through a participatory process; affliction and astute respiratory illness-related affection are calm from association associates who voluntarily abode their bloom cachet on a account base via web and adaptable apps. Abstracts from these systems accept accustomed the abeyant to bigger accept astute respiratory affliction in the citizenry by audition trends aboriginal and at the point of contact, piecing calm real-time patterns of ache spread, anecdotic important subpopulations, ecology the beforehand of an catching or pandemic, and allowance to ambition blockage and ascendancy efforts. However, none of these Internet-based systems apply atomic affection to rapidly affirm the viral account of illness, which is important because the analytic presentation of affliction is agnate to affliction acquired by added respiratory pathogens7.

New apprehension technologies are rapidly arising for home-based and point of affliction diagnosis; they accouter real-time polymerase alternation acknowledgment as able-bodied as newer techniques for aerial acuteness and specificity atomic detection8,9. In all of these technologies, there are accepted accoutrement defective investigation, with account to the types of specimens, both in commendations to achievability of viral apprehension as able-bodied as the alertness and adequacy of individuals to accomplish the sample on their own. Specimens for atomic apprehension of astute respiratory infections about abide of nasopharyngeal ablution and aspirate as the gold standard, about there are assurance apropos for lay volunteers to aggregate this blazon of case at home10. Nasal swabs accept been auspiciously acclimated by volunteers to aggregate applicable samples for affliction adeptness at home, at aboriginal for individuals whom were classified by nurses to accept ‘colds and flu’ 11,27. Further, nasal cocky sampling has been acclimated as allotment of population-based surveillance of respiratory virus infections and compared to ancillary analytic surveillance specimens and accepted practitioner sampling in some European cohorts 26,28. To date, none of these systems accept advised added case types, or appear how individuals’ affection were affiliated to detected infection.

While nasal swabs are accessible to use, the non-invasive attributes of articulate case accumulating makes it an easier and added acceptable and appropriately bigger another to nasal swabs15,16. Articulate specimens accept been broadly acclimated to admission abiogenetic actual for direct-to-consumer medical tests14. As well, although the abstract is sparse, articulate specimens accept been apparent to be reliable in the apprehension of assertive respiratory pathogens. Saliva were begin to be authentic and reliable in the apprehension of H1N1 and commensurable to the nasopharyngeal swab18. Articulate specimens accept additionally been begin to be able in the apprehension of SARS, measles and beta-hemolytic streptococcus in pediatric patients12,13,15,16,17. Others accept begin articulate specimens to be inferior to nasal swabs in the apprehension of respiratory viruses, but still acceptable in settings breadth accepting a nasal besom would be impractical21. Above case type, association accord in appraisal and case accumulating for flu surveillance is additionally unexplored. Academic and bartering entities accept demonstrated19,20,21 that home sample accumulating is achievable from the association and at calibration for genomic, microbiome and STD testing23,24, about the account and architecture of what advice should be announced aback to individuals, in adjustment to be advantageous to appulse accessible bloom behaviors is a accompanying and accessible question.

In sum, while there accept been some efforts to use nasal swabs for self-collection of specimens and surveillance of astute respiratory infection at scale, the assignment appear actuality adds to these absolute efforts by because important questions of account of altered case types, bond of alone participatory evidence letters to case types, and how the constant surveillance advice can be acclimated at an alone level. To abode these needs, we developed the GoViral platform. This belvedere is advised to admission association generated analytic samples and brace them with added ancillary advice such as participatory syndromic reports. Thus, GoViral enables us to test: 1) the types of samples that individuals are able to and accommodating to accomplish in their own homes in a adapted appearance aback they accept an astute respiratory infection forth with and adapted processing methods, 2) how user-generated analytic advice corresponds to self-reported affection and 3) accession and use of the advice by the public. Herein we abode after-effects from an antecedent deployment during the 2013-2014 affliction season

Actor Application and Sign Up

A accomplice of lay volunteers was recruited in Massachusetts. Application was accessible throughout the abstraction aeon (November 1, 2013 to April 30, 2014). We acclimated a accessibility sample in adjustment to rapidly recruit participants and appraise the belvedere during this aboriginal division (thus anticipating participants to be skewed appear those who accept admission to Internet), acceptance appraisal via a added adumbrative sampling during consecutive years. Enrollment was apprenticed abundantly by announcements on bounded radio stations and chat of mouth. Paid online advertisements and amusing media were additionally acclimated as agency of recruiting volunteers to the study. The abstraction admeasurement was bound by our adeptness to recruit and appoint participants. In adjustment to register, volunteers active an cyberbanking accord anatomy and appear their email address, name and commitment address, gender and age. Volunteers were anon beatific a kit (see breadth below) to accumulate at home. Users were instructed that if they became ailing with affection of a algid or flu, they should accomplish the case accumulating and accelerated assay aural 48 hours of experiencing symptoms. Participants who alternate a kit were beatific a replacement, acceptance them to abode a additional affliction (second affliction was included if the case was provided at atomic 2 weeks afterwards the antecedent specimen). GoViral participants were additionally registered for Flu A You and appear affection through account surveys, as has been ahead described6. If a actor appear any affection on their account survey, they anon were beatific an email admonition to chase the instructions in the kit and abide specimens. Instructions for acquiescence of the kit were fabricated accessible on a abode page. On the abode page, an alone confirms the affection that they have, can appearance video instructions (Supplementary Information) for anniversary of the sample types and accelerated test, and they almanac the date affliction began, the calculation on their sample tubes, and assay after-effects from the accelerated test. All participants who active up with a Massachusetts commitment abode were beatific a kit and their evidence letters were included in the study.

Ethics

The agreement was accustomed by the Boston Children’s Hospital Institutional Review Board.

Kit Contents

Each kit (Figure 1) included an Oragene OM-505 Saliva accumulating tube (DNA Genotek Inc.), flocked nasal besom with viral accumulating media (part cardinal 407C, Analytic Hybrids), and a crabbed breeze immunoassay accelerated assay (CerTest Biotec). The CerTest accelerated assay activated for Affliction A, Affliction B, RSV, and adenovirus. For the nasal specimen, we called a nasal besom rather than a nasopharyngeal besom because it is easier to aggregate for the individual, and a antecedent abstraction has accustomed that they are commensurable in acuteness aback application atomic analytic tests13. Self-collected flocked swabs accept additionally been begin to be agnate in corpuscle crop compared to agents calm flocked swabs22 and respiratory corpuscle yields were college aback application flocked nasal swabs compared to rayon swabs14. For the saliva sample, we acclimated the Oragene Saliva accumulating tube which has been advised for the accumulating and stabilization of microbial DNA and RNA from saliva. Samples are appear to abide abiding at allowance temperature for up to 3 weeks in the adherence band-aid included in the tube23. The kits, forth with prepaid postage were mailed to participants at the alpha of the affliction division with pre-paid acknowledgment brief postage. We acclimated USPS so that participants artlessly had to acknowledgment the kits by bottomward them off in any mailbox or column office. Kits were commutual with customized accounting instructions and advisory videos (see case accumulating section).

Fig. 1: Photo of case accumulating kit with components. Anniversary of the kits beatific to participants included a a) accelerated test, b) accelerated assay aqueous reagent, c) nasal besom tube with carriage medium, d) articulate Case beneficiary and e) flocked nasal swabs.

Case Collection

Diagnostic Accuracy of Rapid Antigen Detection Tests for Respiratory ..
Diagnostic Accuracy of Rapid Antigen Detection Tests for Respiratory .. | rapid rh test results form

Participants were instructed to aggregate a nasal sample by inserting the flocked nasal besom about 2.5 cm into one nostril and rotating. The besom was again placed in carriage media for testing. 2 mL of saliva in carriage media was collected. A additional nasal besom alloyed with reagent was calm and again the reagent was acclimated as per instructions for the accelerated assay 27. From the accounting GoViral kit instructions participants were directed to the GoViral website to abide evidence information, kit number, and photo of accelerated assay results. Customized YouTube advisory videos (https://www.youtube.com/watch?v=GWRMbvFjRsg, https://www.youtube.com/watch?v=jAQ-OF8-cyA, https://www.youtube.com/watch?v=S1oE4DLDiw8) showed participants how to aggregate the articulate and nasal specimens and amalgamation them for mailing. Samples were beatific by brief mail to the class for detection.

Case Preparation and Analysis

Specimens accustomed were afar (both the brace of nasal and saliva specimens) if they were accustomed and had leaked due to poor packaging or appeared to not accept any saliva added. We activated samples on both the GenMark Respiratory Viral Panel, and Biofire FilmArray Respiratory Panel. Both action a circuitous console for respiratory pathogens. They about alter in the DNA/RNA abstraction methods used, in the abstinent acuteness and specificity (for nasopharyngeal specimens), and workflow parameters, such as hands-on time, time to result, and about affluence of use. Antecedent studies accept compared these atomic circuitous platforms including class developed atomic methods (PCR)25. While all of these studies were application nasopharyngeal (NP) besom specimens, because of the altered nucleic abstraction methods in anniversary belvedere and abeyant differences in viral amount and breadth in saliva against nasal specimens, their achievement on these sample types is still indeterminate.

Apprehension on Genmark

Prior to apprehension on the Genmark assay, DNA/RNA were extracted from the samples application the MagNA Pure abstraction protocol. Details of this and added action are appear in the Supplementary Advice section. The Genmark Console includes: Affliction A (Subtype to Affliction A H1, Affliction A H3 and Affliction A 2009 H1N), Affliction B, Respiratory Syncytial Virus (RSV) A, Respiratory Syncytial Virus (RSV) B, Parainfluenza Virus (PIV) 1, Parainfluenza Virus (PIV) 2, Parainfluenza Virus (PIV) 3, Animal Metapneumovirus (hMPV), Animal Rhinovirus (HRV), Adenovirus B/E and Adenovirus C. Viral targets in the FilmArray are Affliction A (Influenza A H1, Affliction A H3, Affliction A 2009 H1N1), Affliction B, Respiratory Syncytial Virus (RSV), Coronavirus HKU1, Coronavirus NL63, Coronavirus 229E, Coronavirus OC43, Parainfluenza Virus (PIV) 1, Parainfluenza Virus (PIV) 2, Parainfluenza Virus (PIV) 3, Parainfluenza Virus (PIV) 4, Animal Metapneumovirus (hMPV), Animal Rhinovirus/Enterovirus and Adenovirus. The aftereffect is not binary, a beginning aeon (C­­t ) of 3.0, bent through acquaintance with the Genmark system, is acclimated to allocate a antibody as detected or not in our analysis.

Apprehension on BioFire FilmArray

Specimens were candy afterward the action begin in the FilmArray Respiratory Console Instruction Booklet. The FilmArray RP contains its own abstraction action and centralized controls aural anniversary pouch. In the FilmArray, DNA/RNA is extracted from the sample through concrete agitation and actinic disruption of the beef with beads. Possible after-effects accommodate Detected, Not Detected, Equivocal (Influenza A only) and Invalid.

Survey

In adjustment to barometer if analytic assay after-effects or flu accident absolutely access individuals to change behaviors, participants were beatific a assay at the achievement of the abstraction period. One of two versions of the assay was beatific depending on whether the actor had beatific in analytic samples. Participants were asked about their bactericide behaviors and the adversity of the case accumulating protocols (all questions and responses in Table S1).

Outcomes and Statistical Methods

Analyses were performed application R adaptation 2.15.1 (R Development Core Team, Vienna, Austria). Key outcomes of absorption were self-reported affection (participants could abode any of fever, cough, abscessed throat, conciseness of breath, chills, fatigue, nausea, diarrhea, anatomy aches and headache), and analytic after-effects for anniversary of the nasal and saliva specimens via both the Genmark and BioFire systems for anniversary set of specimens beatific in. Timing of case accumulating compared to evidence access was acclaimed in adjustment to accept the abeyant of self-collection. Univariate comparisons amid affection and analytic after-effects were performed, but sample admeasurement was too low to accreditation added analyses.

Actor Demographics and Participation

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Between November 1, 2013 and April 30, 2014, 295 participants enrolled in the abstraction and accustomed a kit. During the abstraction aeon we additionally beatific a additional kit to participants who already completed one kit, acceptance for additional illnesses to be captured. Among the accomplice of enrolled abstraction participants, 167 individuals appear any affection during the abstraction time frame, and from those individuals 71 pairs of analytic samples were alternate (1 alternate nasal sample had no accompanying saliva sample), and beatific for nucleic acerbic analysis. Participants submitted kits an boilerplate of 3.29 (95% CI: 2.39, 4.18) canicule afterwards evidence access (Figure 2). Bodies ages 20 to 77 active up and accustomed kits (Figure 3), and 70% of signups were women. Kits were alternate by individuals alignment in age from 20 to 70.

Fig. 2: Canicule amid evidence access and case submission. The breadth of time was computed based on self-report of affliction access date by the actor and the date they completed a case acquiescence form.

Fig. 3: Gender and ages of participants. Demographics of all individuals who active up as participants, those who concluded up abiding a kit or did not.

Bacilli Detected (BioFire FilmArray, Genmark and accelerated tests)

Out of the 71 pairs of samples tested, 35 sets of samples activated absolute in at atomic one case on both systems, 31 were abrogating on both and there were 5 cases in which both the saliva and nasal specimens activated on one arrangement did not ascertain a virus detected in at atomic one case application the added system: Affliction A was detected in one nasal case by Genmark (and not on the FilmArray), Coronavirus was detected in one saliva sample detected by Genmark (and not on the FilmArray), Coronavirus detected in one nasal sample by FilmArray (and not on the Genmark), Coronavirus detected in one saliva sample by FilmArray (and not the Genmark), RSV was detected in both a nasal and saliva samples from one being on the FilmArray (and not on the Genmark). The administration of samples and bacilli detected in at atomic one case (saliva or nasal) on both systems are illustrated in Figures 4a and b. In 10 cases, there was a aberration amid after-effects for the nasal and saliva case pairs on at atomic one of the systems, but the aforementioned antibody was additionally detected in the added arrangement (Table S2).

Fig. 4: Aggregate after-effects by pathogen. Overall results, as a admeasurement of all participants that had a absolute aftereffect in either case on both systems, for the a) Genmark and b) BioFire FilmArray.

Rapid immunoassays performed by the users were not advantageous in anecdotic the specimens that were absolute with influenza, RSV or adenovirus. None of the accelerated tests alternate alongside any of the 71 alternate pairs of specimens accustomed absolute after-effects for any pathogens, alike ones that were bent absolute by the PCR-based methods.

Accord Amid Affection and Analytic Results

Of bodies who appear The CDC (Centers for Ache Ascendancy and Prevention)’s “influenza-like illness” (ILI) evidence contour (fever and ahem or abscessed throat) two out of twelve of activated absolute for influenza, bristles out of twelve did not assay absolute for annihilation on the console and bristles out of twelve activated absolute for added bacilli (Coronavirus, hMPV and RSV). The abounding arbitrary of evidence profiles by antibody is provided in Table S3.

Actor Assay Results

Results of the assay are appear in Table S1. Overall, it was begin that i) best participants who beatific in a kit did not seek any interventions from healthcare professionals for their affliction (86.7%), ii) it was harder for bodies to aftermath the nasal sample than the saliva sample, and iii) above award out their own diagnosis, best individuals appear that they would acquisition it additionally advantageous to apperceive what others in their breadth accept had a them (83.3% for those who beatific in kits, 93.7% for those who did not).

Our abstraction seeks to accurately abstraction user preferences and case types appear architecture a scalable adjustment aing self-reported affection to infection blazon for compassionate association prevalence of respiratory infections. Assorted antecedent allegation are offered: analytic samples can be generated by the accessible accustomed by the aerial user accord amount (half of the bodies who appear any affection at all completed the kit and beatific in specimens), and antecedent validation of articulate specimens as compared with nasal specimens can be acclimated for the identification of aerial respiratory pathogens, forth with letters of greater abundance and alternative for articulate specimens. While we ahead the accord amount is biased upwards based on the types of bodies who may accept agreed to participate in the study, it indicates that while ailing at home, bodies are accommodating and able to complete the case collection. Affliction A, RSV, Coronavirus (3 subtypes), hMPV and HRV were detected from saliva and nasal samples, with aerial acceding amid the nasal and saliva samples abstinent on two altered apprehension methods. Pairing evidence profiles with the detected bacilli showed that best bodies who self-report the accepted ILI contour did not accept influenza. Through our survey, we begin that best individuals who became ill and alternate a sample did not seek healthcare, such as activity to a dispensary or the hospital. Overall, saliva accumulating was appear to be easier than the nasal besom case collection. Accelerated immunoassays did not appearance amount for association use, compared to the PCR apprehension of community-acquired specimens. Above the accepted lower acuteness and specificity of the accelerated tests, the blueprint for these tests accept been bent application nasopharyngeal swabs on children, appropriately the achievement on specimens from adults and application nasal swabs can be attributed to this difference. The altered assays, GenMark and BioFire FilmArray, accustomed agnate achievement with some apocryphal negatives on both saliva and nasal specimens from anniversary arrangement as compared to the other. We additionally begin that individuals would acquisition it advantageous to apperceive what bacilli were detected in added bodies in the community. Regarding award out their own diagnosis, best accepted affidavit cited for acquisitive this advice included affair for acknowledgment to others, abnormally by those who additionally mentioned they assignment in healthcare. The after-effects presented actuality beforehand methods for accomplishing these preferences, and with added studies involving added and assorted groups the ability of these abstracts will increase.

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There are abounding aspects of the abstraction which can be bigger and approaching assay should address. One limitation of this abstraction was the baby and non-representative sample of volunteers. Based on the Internet or adaptable claim for participation, as able-bodied as a self-selective attributes of interest, participants of this abstraction are agnate demographically to those in added participatory systems6. Geographically, application was agilely bound but will be broadcast in the approaching to ambition under-represented groups. Although the cardinal of samples accustomed was constant with the accepted cardinal of individuals who would be ailing with some blazon of respiratory infection, this indicates that in adjustment to access the cardinal of samples, a greater cardinal of participants are required. Finally, while accelerated tests were included in the kits and accepting accelerated after-effects is an allurement for individuals to participate, the achievement was not specific nor astute abundant to accreditation added use. Altered accelerated analytic tests may be experimented with in accessible kits, as while the majority of individuals abode they would like to accept the after-effects of their own tests aural two days, which is achievable with this model, a added real-time assay is optimal for accommodation making.

This abstraction represents an beforehand with account to the acreage of participatory accessible bloom surveillance systems. To-date, letters in the abstract of online participatory studies accept been bound to self-reported syndromic advice only. Some studies accept accumulated self-reported affliction affection with serology to accept advance ante in the population. However, bond to affection has been bound in self-sampling studies, and although added studies accept accustomed that individuals can admission a nasal besom case from themselves, we abode actuality that individuals adopt saliva and antecedent after-effects announce that it can potentially accommodate a achievable boilerplate for astute aerial respiratory infection detection. Finally, via the after-effects of our survey, it is adumbrated that participatory advice (crowdsourced) can be acclimated to accomplish context-specific, accelerated advice for ache surveillance that has the abeyant to appulse accessible bloom behaviors.

Going forward, added abstraction of abstracts from the GoViral surveillance adjustment offers abeyant allowances such as compassionate domiciliary manual characteristics, enabling appraisal of the accord amid appear evidence profiles and viral assay as able-bodied as spatio-temporal administration of aerial respiratory infection, continuance of affection and its affiliation to viral etiology, and appulse of these efforts on an individual’s accessible bloom and healthcare gluttonous behavior. Above a accomplice access here, association case accumulating can be acclimated during melancholia beginning or catching investigations. Approaching assignment will access the cardinal and representatively of abstraction actor demographics, as able-bodied as apply next-generation apprehension technologies to advance acuteness and acceleration of breeding the analytic results.

All authors accept accepted that they accept no financial, claimed or able aggressive interests to report.

1. Do you booty any bactericide measures during flu division (multiple choices allowed)?

Beatific in kits (n = 29 responses)

Work/studying from home 3 (10%) Extra duke abrasion 18 (62%) Got a vaccine 19 (65%) Avoiding acquaintance with others 8 (28%) Didn’t booty any 2 (7%)

Did not accelerate in kits (143 responses)

Work/studying from home 14 (10%) Extra duke abrasion 63 (44%) Got a vaccine 106 (74%) Avoiding acquaintance with others 20 (14%) Didn’t booty any 21 (15%)

2. Did your affliction this division for which you beatific in a sample ahead you (or the domiciliary affiliate who’s case it was) from accommodating in any accustomed activities (work, school) If yes, for how abounding days?

Beatific in kits (28 responses)

No 12 (43%) 1-2 canicule 10 (36%) 3 canicule 3 (11%) 1 anniversary 1 (4%) 10 canicule 1 (4%) Can’t bethink 1 (4%)

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3. Did you go see a doctor, or go into the hospital or seek some affectionate of affliction because of your illness?

Beatific in kits (30 responses)

4. What allotment of the action of appointment your sample was easiest?

Beatific in kits (29 responses)

Filling out the online anatomy 20 (69%) Application nasal besom 4 (14%) Saliva accumulating 5 (17%)

5. What allotment of the action of appointment your sample was hardest?

Beatific in kits (26 responses)

Filling out the online anatomy 5 (20%) Application nasal besom 12 (46%) Saliva accumulating 9 (35%)

6. Would you acquisition it advantageous to apperceive the aftereffect of what you had aback alpha activity sick?

Beatific in kits (30 responses)

Aural 1 day 11 (37%) Aural 2 canicule 9 (30%) Aural a anniversary 4 (13%) Just award out at end of division is accomplished 6 (20%)

Did not accelerate in kit (138 responses)

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A comparison of effectiveness between oral rapid testing and routine .. | rapid rh test results form

Aural 1 day 34 (25%) Aural 2 canicule 33 (24%) Aural a anniversary 26 (19%) Just award out at end of division is accomplished 45 (33%)

7. Would you acquisition it advantageous to apperceive what others in your breadth accept had a you (such as in the map?)

Beatific in kits (30 responses)

Did not accelerate in kit (141 responses)

8. What would you do with this information?

Beatific in kits (27 responses)

Just for information/curiosity 6/27 (22%) Booty adapted precautions for self/others 11/27 (41%) Tell doctor 3/27 (11%) Doctor and activities 5/27 (19%) Nothing 2/27 (7%)

Didn’t accelerate in a kit (110 responses)

Just for information/curiosity 34/110 (31%) Booty adapted precautions for self/others 51/110 (46%) Tell doctor 14/110 (13%) Doctor and activities 3/110 (3%) Nothing 8/110 (7%)

Sample Blazon Genmark Aftereffect FilmArray Aftereffect Nasal Infl A 2009 H1N1 Ct=136.2Infl A Ct=115 none Saliva Infl A 2009 H1N1 Ct=137.3 FLA Nasal abrogating none Saliva abrogating none Nasal Corona 229E Ct=226.3 CORONA Saliva Corona 229E Ct=141.4 CORONA Nasal abrogating none Saliva abrogating none Nasal abrogating none Saliva abrogating none Nasal Corona 229E Ct=264.2 CORONA Saliva Corona 229E Ct=235.7 CORONA Nasal Infl A 2009 H1N1 Ct=347Infl A Ct=201.3 FLA Saliva abrogating none Nasal abrogating none Saliva abrogating none Nasal Corona HKU1 Ct=243.3 CORONA Saliva Corona HKU1 Ct=279 CORONA Nasal abrogating none Saliva abrogating none Nasal abrogating EN-RH Saliva abrogating EN-RH Nasal Corona NL63 Ct=6.9 CORONA Saliva Corona NL63 Ct=16.5 CORONA Nasal Infl A 2009 H1N1 Ct=348Infl A Ct=155 FLA Saliva Infl A 2009 H1N1 Ct=80.2Infl A Ct=208 FLA Nasal Infl A 2009 H1N1 Ct=210Infl A Ct=225 FLA Saliva abrogating none Nasal Infl A 2009 H1N1 Ct=160Infl A Ct=97.6 none Saliva abrogating FLA Nasal Corona 229E Ct=9.1 none Saliva Corona 229E Ct=5.8 CORONA Nasal abrogating none Saliva abrogating none Nasal Corona HKU1 Ct=269 CORONA Saliva Corona HKU1 Ct=240 CORONA Nasal abrogating none Saliva abrogating none Nasal RSV A Ct=176 RSVI Saliva RSV A Ct=152 RSVI Nasal abrogating none Saliva abrogating CORONA Nasal Corona 229E Ct=202Corona HKU1 Ct=279 CORONA Saliva Corona 229E Ct=4.3Corona HKU1 Ct=169 CORONA Nasal abrogating none Saliva abrogating none Nasal Corona 229E Ct=197.2 CORONA Saliva Corona 229E Ct=206.5 CORONA Nasal abrogating none Saliva abrogating none Nasal abrogating none Saliva abrogating none Nasal abrogating none Saliva abrogating none Nasal abrogating none Saliva abrogating none Nasal Corona 229E Ct=206 CORONA Saliva Corona 229E Ct=207 CORONA Nasal Corona HKU1 Ct=184 none Saliva abrogating CORONA Nasal abrogating none Saliva abrogating none Nasal Corona HKU1 Ct=153 CORONA Saliva abrogating none Nasal abrogating none Saliva abrogating none Nasal abrogating none Saliva abrogating none Nasal Corona HKU1 Ct=213 CORONA Saliva abrogating none Nasal abrogating none Saliva abrogating none Nasal abrogating none Saliva abrogating none Nasal abrogating none Saliva abrogating none Nasal abrogating none Saliva abrogating none Nasal CORONA NL63 Ct=5.3 CORONA Saliva abrogating CORONA Nasal abrogating CORONA Saliva abrogating none Nasal abrogating none Saliva abrogating none Nasal abrogating none Saliva abrogating none Nasal abrogating none Saliva abrogating none Nasal abrogating none Saliva abrogating none Nasal abrogating none Saliva abrogating none Nasal Infl A 2009 H1N1 Ct=229Infl A Ct=233 FLA Saliva Infl A 2009 H1N1 Ct=312Infl A Ct=253 FLA Nasal abrogating none Saliva abrogating none Nasal Corona HKU1 Ct=3.8 none Saliva Corona HKU1 Ct=91.4 CORONA Nasal abrogating none Saliva abrogating none Nasal Corona 229E Ct=105 none Saliva Corona 229E Ct=197 CORONA Nasal abrogating none Saliva abrogating none Nasal abrogating none Saliva HRV Ct=180 EN-RH Nasal abrogating none Saliva Corona 229E Ct=5.8 none Nasal abrogating none Saliva abrogating none Nasal abrogating none Saliva abrogating none Nasal RSV A Ct=201 RSVI Saliva RSV A Ct=137 RSVI Nasal Corona NL63 Ct=16.5 CORONA Saliva abrogating CORONA Nasal HRV Ct=17.1 EN-RH Saliva HRV Ct=25.4 EN-RH Nasal abrogating none Saliva abrogating none Nasal Infl A H3 Ct=148 Infl A Ct=32.4 FLA Saliva Infl A H3 Ct=161 Infl A Ct=46.1 FLA Nasal abrogating none Saliva abrogating none Nasal Corona 229E Ct=167 CORONA Saliva Corona 229E Ct=48 CORONA Nasal hMPV Ct=140 HMPV Saliva hMPV Ct=120 HMPV Nasal HRV Ct=14.6 EN-RH Saliva HRV Ct=9.6 EN_RH Nasal Infl A H3 Ct=81.6 Infl A Ct=12 none Saliva abrogating none Nasal Infl A H3 Ct=147 Infl A Ct=67.6 FLA Saliva abrogating none Nasal HRV Ct=157 EN-RH Saliva HRV Ct=7 EN-RH Nasal HRV Ct=212 EN-RH Saliva HRV Ct=148 EN-RH Nasal abrogating none Nasal HRV Ct=141 EN-RH Saliva HRV Ct=128 EN-RH Nasal HRV Ct=26.7 EN-RH Saliva HRV Ct=17.3 EN-RH

Affection Appear Affliction A (8) Coronavirus (17) HMPV (1) HRV (7) RSV (2) Abrogating (31) agitation alone (1) 0.0 0.00 0.00 0.0 0.0 3.2 ahem alone (7) 25.0 5.9 0.00 28.6 0.0 6.5 abscessed throat alone (16) 12.5 41.2 0.00 28.6 0.0 19.4 none (19) 0.0 23.5 0.00 14.3 0.0 45.2 ahem and abscessed throat (11) 37.5 11.8 0.00 28.6 50.0 9.7 fever, ahem and abscessed throat (4) 0.0 5.9 100.0 0.00 0.0 6.5 agitation and ahem (4) 25.0 0.00 0.00 0.00 50.0 3.2 agitation and sorethroat (4) 0.00 11.8 0.00 0.00 0.0 6.5

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